T2885

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T2885

Sigma

 

3,3′,5,5′-Tetramethylbenzidine

≥98% (TLC)

Synonym:TMB
CAS Number:54827-17-7
Linear Formula:[-C6H2(CH3)2-4-NH2]2
Molecular Weight:240.34
Beilstein Registry Number:2808541
EC Number:259-364-6
MDL number:MFCD00007748
PubChem Substance ID:24900090

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Description

Application3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8

Properties

assay≥98% (TLC)
mp168-171 °C(lit.)
storage temp.2-8°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXi
Risk Statements36/37/38
Safety Statements26-36
WGK Germany3
RTECSDV2300000

References

Cited Reference1. Holland, V.R., et al. Tet. 30, 3299, (1974)
 2. D.D. Garner et al. J. Forensic Sci. 21, 819, (1976)
 3. J.C. Standefer, D. Vanderjagt Clin. Chem. 23, 749, (1977)
 4. R.C. Lijana, M.C. Williams J. Lab. Clin. Med. 94, 266, (1979)
 5. Liem, H.H. et al. Anal. Biochem. 98, 388, (1979)
 6. Bos, E.S., et al. J. Immunoassay 2, 187, (1981)
 7. P.C. Andrews, N.I. Krinsky Anal. Biochem. 127, 346, (1982)
 8. H. Gallati, I. Pracht J. Clin. Chem. Clin. Biochem. 23, 453, (1985)
referenceAldrich MSDS 1, 1679:B / Corp MSDS 1 (2), 3292:A / FT-IR 2 (2), 2090:C / FT-IR 1 (1), 1236:D / FT-NMR 1 (2), 529:B / FT-NMR 1 (1), 1236:D / IR-Spectra (3), 740:D / RegBook 1 (1), 1423:J / Sigma FT-IR 1 (2), 218:D / Stains and Dyes Ref, 693 / Structure Index 1, 222:D:3